Table 1.

Ability of various glycosphingolipids to recognize the pH 6 antigen

Structure of glycosphingolipidaName or designation of glycosphingolipidbResult of binding after separation by TLC with:Result of binding in an assay based on ELISAc
pH 6 antigenE. coli/pDG1
Galβ1-1Cer (hydroxylated fatty acids)Galactosylceramide++N/A
Galβ1-1Cer (nonhydroxylated fatty acids)Galacosylceramide+N/A
Galβ1-1CerGlucosylceramideN/A
Galβ1-4Glcβ1-1CerLC+++
GalNAcβ1-4Galβ1-4Glcβ1-1CerGM2A+++
Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-1CerGM1A+++
Galα-4Galβ1-4Glcβ1-1CerGlobotriaosylceramide
GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1CerGlobotetraosylceramide (globoside)
GalNAcβ1-3GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1CerGlobopentaosylceramide (Forssman glycolipid)
Galβ1-3GalNAcβ1-4Gal3→2NeuAc)β1-4Glcβ1-1CerGM1
GalNAcβ1-4Gal(3←2NeuAc)β1-4Glcβ1-1CerGM2
NeuAcα1-3Galβ1-4Glcβ1-1CerGM3
NeuAcα2-3Galβ1-3GalNAcβ1-4Gal(3←2αNeuAc)β1-4Glcβ1-1CerGD1a
Galβ1-3GalNAcβ1-4Gal(3←2αNeuAc8←2αNeuAc)β1-4Glcβ1-1-CerGD1b
GalNAcβ1-4Gal(3→2NeuAc8←2αNeuAc)β12-4Glcβ1-1CerGD2
NeuAcα2-8NeuAcα2-3Galβ1-4Glcβ1-1CerCD3
NeuAcα2-8NeuAcα2-3Galβ13GalNAcβ1-4Gal(3←2NeuAc8←2αNeuAc) β1-4Glcβ1-1CerGQ1b
NeuAcα2-3Galβ1-3GalNAcβ1-4Gal(3β1-4Glcβ1-1CerGT1b
  • a Underlining indicates minimum binding determinant.

  • b Abbreviations for gangliosides follow the nomenclature system of Svennerholm (17).

  • c Purified glycosphingolipids and enzymes were obtained from Sigma Chemical Co. (Poole, United Kingdom).

  • d An OD414 of >0.1 above those of controls was considered a positive result. N/A, not applicable.