TABLE 1.

Bacterial strains and plasmids used in this study

Plasmid or strainDescriptionSource or reference
Klebsiella pneumoniae
    LM21Wild-type strain; Apr9
    C-81LM21 with mini-Tn5 inserted in eefA; Apr, Kmr17
    LM21SptLM21 ΔSHV1::aadA7; SprThis study
    LM21ΔeefALM21 ΔSHV1::aadA7 ΔeefA::aph3; Spr, KmrThis study
    LM21ΔeefAAprLM21 ΔeefA::aph3; Kmr, Apr, Spr (spontaneous mutant)This study
    LM21ciseefXALM21 ΔSHV1::Φ (eef-eefX-eefA-aadA7) ΔeefA::aph3; Spr, KmrThis study
    Φ (eef-lacZ)LM21 ΔeefA::lacZ-aph3; KmrThis study
    LM21CH199LM21 containing the pKOBEG199 plasmid; Apr, Tcr
Escherichia coli K-12
    JM109F′ traD36 proA+B+ lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14 gyrA96 recA1 relA1 endA1 thi hsdR17
    C600F thr-1 leuB6 thi-1 lacY1 glnV44 rfbD1 fhuA2127
    CH158Pop3ybeW::aph35
Plasmid
    pKOBEG199Plasmid with λ red genes under the control of the arabinose-inducible promoter2