Table 3.

Expression of I-Ad/I-Ed molecules on F4/80+ or CD11b+ spleen cells from normal uninfected mice and from infected mice treated with NRG or anti-IL-12 antibodies

Mouse groupMean fluorescence intensity (FL2) of I-Ad/I-Edona:
F4/80+cellsCD11b+ cells
Day 7Day 13Day 7Day 13
Normal91 ± 1382 ± 524 ± 220 ± 1
NRG treated750 ± 501,163 ± 233421 ± 45615 ± 76
Anti-IL-12 treated146 ± 1375 ± 1661 ± 719 ± 9
  • a F4/80+ or CD11b+ cells were gated by using FL1. The results are expressed as the geometric means of fluorescence intensity (FL2). The data represent the means ± standard deviations of three similar experiments. The geometric mean of fluorescence intensity when staining with the conjugated isotype control antibodies was less than 10 for all three groups of mice.