Table 1.

ORFs on pCD1 of Y. pestis KIM5a

Geneb or ORFFunction of productOrientationBeginning of ORF (bp)End of ORF (bp)No. of amino acids in productIsoelectric point of productMass of product (kDa)
repB(copB)Negative regulator of repAtranscription+11711425859.729.58
tap Required for translation ofrepA +16671741259.312.82
repA Plasmid replication+1734260028910.9633.55
Orf5Unknown36453427739.968.22
Orf7Unknown+475851861434.3915.78
ypkA (yopO)Targeted effector; Ser Thr kinase+520474027336.5381.74
yopJ(yopP)Targeted effector; causes apoptosis in macrophages and interferes with cell signaling+779886642897.0732.46
yopH Targeted effector; protein tyrosine kinase; interferes with cell signaling at focal adhesions+10347117534698.6850.87
lcrQ(yscM)Negative regulator of LCR expression16148158011166.3412.41
yscL Type III secretion component17038163732224.5724.65
yscK Type III secretion component17613169842106.7523.99
yscJ Type III secretion component18347176132457.4327.04
yscI Type III secretion component18701183541164.4712.67
yscH(yopR)Secreted; unknown function19199187021665.1418.35
yscG Type III secretion component19543191961166.6013.07
yscF Type III secretion component1980819545887.139.49
yscE Type III secretion component2000919809677.317.61
yscD Type III secretion component21265200064205.8546.93
yscC Type III secretion component23085212626086.4967.35
yscB Unknown23504230911389.2715.41
yscA Unknown2382823730339.823.86
lcrF (virF)Activator of LCR gene expression24722239072728.9130.84
yscW (virG)YscC lipoprotein chaperone252412484613210.1214.71
yscU Type III secretion component26881258173558.8140.39
yscT Type III secretion component27666268812625.6728.45
yscS Type III secretion component2792927663896.329.57
yscR Type III secretion component28584279312184.6824.43
yscQ Type III secretion component29504285813085.0834.42
yscP Type III secretion component30868295014565.4450.42
yscO Type III secretion component31332308681557.8419.00
yscN Type III secretion component32648313294406.4847.81
lcrE(yopN)Secretion control+32846337272945.0732.67
tyeA Secretion and Yop targeting control+3370833986934.2110.75
Orf42Unknown+33973343441245.5413.61
Orf43Unknown+34341347091236.3213.76
Orf44Unknown+34706350501156.9213.12
lcrD(yscV)Secretion+35037371517055.0477.81
lcrR Unknown+371483758814710.2716.46
lcrG Secretion control; efficient Yop targeting+3763037917968.1511.02
lcrV Diffusible effector; secretion and targeting control+37919388993275.6637.24
lcrH(sycD)YopB and YopD chaperone+38912394181694.6119.02
yopB Yop targeting+39396406014027.0941.83
yopD Yop targeting; negative regulator+40620415403076.8033.39
Orf54Unknown42709423861089.6612.61
yopM Targeted effector+43481447104104.2346.21
Orf60Unknown46365459461407.7915.81
Orf61Unknown+46637470261307.3314.80
sycT YopT chaperone47468470701334.4315.42
yopT Targeted effector48436474683239.1336.31
yopK(yopQ)Yop targeting+48936494841834.3721.00
ylpA Pseudogene+50089507182105.8022.40
sopA Plasmid partitioning; negative regulator ofsopABtranscription+52730538963895.8243.41
sopB Plasmid partitioning; binds to sopCregion+538965485832010.1935.61
Orf73Unknown+5608756362926.1610.10
Orf74Unknown+56355566541005.5111.67
Orf75Unknown5679256496999.8811.19
yopE Targeted effector; causes actin depolymerization57453567942206.5922.99
sycE (yerA)YopE chaperone+57647580391314.4914.65
sycH YopH chaperone+60796612211424.8115.76
Orf84Unknown62897625681108.9813.00
Orf85Unknown63500630361554.9717.71
yadA′ Pseudogene+6753267783845.218.92
′yadA Pseudogene+67900688353126.8432.47
  • a ORFs within transposable elements, as well as disrupted or partial ORFs (except for ylpA,yadA′, and ′yadA), are not included in the table.

  • b Except for copB, yopN,yscV, and yerA, all alternate gene designations (in parentheses) are Y. enterocolitica terminology.copB, plasmid R100 terminology; yopN, Y. enterocolitica and Y. pseudotuberculosis terminology;yscV, proposed terminology change; yerA, Y. pseudotuberculosis terminology.