Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference(s)
Strains
 HN280Wild-type EIEC strain of serotype O135:K−:H−; contains virulence plasmid pHN280; apy + 7, 9; this study
 HN280/32pHN280-integrated derivative of strain HN280; noninvasive; apy + 46
 HN280-3 and -8Independent spontaneous Crbderivatives of strain HN280; pINV carries apy,virB, and icsA and lacks virF This study
 HN280-16 and -35Independent spontaneous Crb derivatives of strain HN280; pINV carriesapy, virF, and icsA and lacksvirB This study
 HN680Δ(hns tdk adhE oppABCD)118 zch-506::Tn10derivative of strain HN280; invasive; apy +Tcr 9
 HN680/32Δ(hns tdk adhE oppABCD)118 zch-506::Tn10derivative of strain HN280/32; invasive; apy +Tcr 9
 SFZM53Wild-type invasive S. flexneri strain of serotype 4;apy + phoN-Sf + 6
 SFZM53-1Independent spontaneous Crb derivative of strain SFZM53; pINV carries apy, virB, andicsA and lacks virF andphoN-Sf This study
 SFZM53-11Independent spontaneous Crb derivative of strain SFZM53; pINV carriesapy, phoN-Sf, and virB and lacksvirF and icsA This study
 SFZM53-15Independent spontaneous Crb derivative of strain SFZM53; pINV carries apy, phoN-Sf,virF, and icsA and lacks virB This study
 M90TWild-type invasive S. flexneri strain of serotype 5; apy+phoN-Sf + 34
 BS176pINV-cured derivative of strain M90T; noninvasive 35
 454Wild-type invasiveS. flexneri strain of serotype 2a; apy+phoN-Sf + 2
Plasmids
 pBR322Cloning plasmid vector; AprTcr 33
 pMYSH6504pBR322-derived vector carrying the virFgene; Apr Kmr 32
 pBN1pBR322-derived vector carrying the virB gene; Tpr 1
  • a Tcr, tetracycline resistant; Kmr, kanamycin resistant; Apr, ampicillin resistant; Tpr, trimethoprim resistant.