Table 1.

Strains and plasmids used in this study

Strain or plasmidCharacteristic(s)Source or reference
Strains
 SA100 S. flexneri wild type, serotype 2a, Crb+, virulent 46
 SA101SA100 derivative, deletion in the large virulence plasmid, Crb, noninvasive 9
 SA511SA100 Cmr, invasive, formed normal plaques 21
 SA2122SA511vpsC::TnphoA, Kmr This study
 SA2054SA511ispA::TnphoA, Kmr This study
 SA4100SA100vpsA::aphA-3, Kmr This study
 SA5122SA100vpsC::TnphoA, Kmr This study
 SA5122WSA5122 Crb This study
 W3110 E. coli K-12I. J. Molineux
 SB221 E. coli lpp 36
 SM10λpir E. coli pirR6K 63
Plasmids
 pACYC177cloning vector, Apr Kmr 7
 pACYC184cloning vector, TcrCmr 7
 pBR322cloning vector, Apr Tcr 4
 pWSc-1cloning vector, Sucs 40
 pMQASA100vpsA-yrbA cloned in pBR322, Tcr This study
 pMQBSA100 vpsC-yrbA cloned in pBR322, Tcr This study
 pMQCSA100 vpsA-Ccloned in pBR322, Tcr This study
 pMQDSA100vpsA-yrbC cloned in pBR322, Tcr This study
 pMQESA100 vpsA-yrbB cloned in pBR322, Tcr This study
 pMQFSA100 vpsCcloned in pBR322, Tcr This study
 pMQGSA100vpsA-B cloned in pBR322, Tcr This study
 pYG400 vpsA::aphA-3 cloned in pWSc-1This study
 pRZ526 E. coli yciC-tonBcloned in pRZ112, Kmr 51
 pML14pRZ526 with bla from pACYC177 inserted at theHindIII site, Apr Kmr This study
 pRT733 oriR6K mob+TnphoA AprKmr 63