Table 3.

Expression of nonspecific phosphatase byS. flexneri strains

StrainRelevant genotypeNonspecific phosphatase activitya at:Ratio of apyrase activity (37°C/30°C)
30°C37°C
M90TWild-typeS. flexneri serotype 50.2261.1985.3
BS176pINV curedNDND
SFZM53Wild-type S. flexneri serotype 40.1530.9786.4
SFZM53-11Δ(virF icsA)0.1041.05610.1
SFZM53-15ΔvirB 0.0931.12812.1
454Wild-type S. flexneri serotype 2a0.1360.9426.9
  • a Expressed as AU obtained by scanning of developed zymograms as described in Materials and Methods. Results are averages for at least four independent experiments. Standard errors were less than 15%. ND, not detectable.