Table 1.

Strains and plasmids used

Strain or plasmidDescriptionSource or reference
E. coli strains
 XL1 Blue supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 Lac(F′ [proAB+ lacI q lacZ ΔM15 Tn10 {Tet}]) 5
 HMS174 recA1 hsdRRifr 47
Plasmids
 pUC19Cloning vector, Ampr 54
 pGEM-T EasyCloning vector, Ampr Promega
 pET-28a(+)Cloning vector, Kanr Novagen
 pTK30035.6-kb EcoRI fragment in pUC19This study
 pTK3005Reverse insertion of 5.6-kb EcoRI fragment in pUC19This study
 pTK30223.4-kb SmaI-EcoRI fragment in pUC19This study
 pTK30343.7-kb 3′ deletion fragment of pTK3003 EcoRI insertThis study
 pTK30353.65-kb 3′ deletion fragment of pTK3003 EcoRI insertThis study
 pTK30372.9-kb 3′ deletion fragment of pTK3003EcoRI insertThis study
 pTK30431.9-kb 3′ deletion fragment of pTK3003 EcoRI insertThis study
 pTK30471-kb 3′ deletion fragment of pTK3003 EcoRI insertThis study
 pTK30465.1-kb 3′ deletion fragment of pTK3005 EcoRI insertThis study
 pTK30454.2-kb 3′ deletion fragment of pTK3005 EcoRI insertThis study
 pTK3251 cdtA (1,093 bases) PCR fragment in pGEM-T EasyThis study
 pTK3252 cdtB (1,211 bases) PCR fragment in pGEM-T EasyThis study
 pTK3253 cdtC(969 bases) PCR fragment in pGEM-T EasyThis study
 pTK3286 cdtA (636 bases) PCR fragment in pET-28a(+)This study
 pTK3287 cdtB (797 bases) PCR fragment in pET-28a(+)This study
 pTK3288 cdtC (561 bases) PCR fragment in pET-28a(+)This study