Table 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
S. pneumoniae
  WU2Capsular type 3, PspA type 1 5
  D39Capsular type 2, PspA type 25 1
  R36ANonencapsulated mutant of D39, PspA type 25 1
  Rx1Nonencapsulated variant of R36A, PspA type 25 21
  A66Capsular type 3, PspA type 13 1
  BG7322Capsular type 6, PspA type 24 16
  EF5668Capsular type 4, PspA type 12 16
E. coliDH5αHost strain for plasmids 11
Plasmids
 pJY4163Emr 26
 pKSD2106pJY4163::BamHI-SalI PCR product from EF5668, carries DNA that encodes PspA/EF5668This study