TABLE 1.

Magellan 5 tranposon insertion biofilm mutants

MutantaGenebFunctional annotationbTransposon positionc% Biofilm formationd
30B10 (2)SP0013, or ftsHPutative single-chain AAA protease5034 ± 8
46E5 (2)SP0060Putative glycosyl hydrolase1727244 ± 18
63A5SP0137Putative ABC transporter, ATP binding110854 ± 6
12G2SP0140, or ugdUDP-glucose 6-dehydrogenase, authentic frameshift648192 ± 26
9H2 (5)SP0199, or clsPutative cardiolipin synthetase2050 ± 10
76G6SP0200, or ccs4Putative competence-induced protein62060 ± 5
70E2 (2)SP0391, or cbpFCholine binding protein F72936 ± 6
12D4SP0393Putative IS3-SPN1 hypothetical protein, point mutation106852 ± 31
70B2 (1)SP0461rlrA islet transcriptional regulator69355 ± 10
60F5 (6)SP0462, or rrgAPilus99645 ± 8
40F1SP0615, or fibAPutative beta-lactam resistance factor93847 ± 9
72F3 (2)SP0619Conserved hypothetical protein40038 ± 11
8A10SP0646Putative PTS system, IIB component137337 ± 94
36C1SP0648, or bgaAPutative beta-galactosidase36 (5′)309 ± 143
67C10SP0693Putative hypothetical protein6436 ± 7
5F10SP0694Putative conserved-domain protein46 (5′)33 ± 5
35C8 (3)SP0695Putative HesA/MoeB/ThiF family protein5 (5′)42 ± 5
75F5SP0696Putative hypothetical protein14944 ± 11
61H6SP0698Hypothetical protein64737 ± 12
1E9SP0746ATP-dependent Clp protease, proteolytic subunit (ClpP)58 (5′)48 ± 9
70E9 (3)SP0785Conserved hypothetical protein21753 ± 17
69B2SP0799, or ciaHSensor histidine kinase38862 ± 11
62A2SP0801Conserved hypothetical protein1956 ± 7
56G6SP0975Putative exoribonuclease VacB/Rnb family49860 ± 12
29D8SP0979, or pepFPutative oligoendopeptidase F154053 ± 13
4E1SP1154ZmpA, immunoglobulin A1 protease5580165 ± 38
67F4SP1182, or lacR2Putative lactose PTS repressor279333 ± 83
69H1SP1370, or aroKPutative shikimate kinase10545 ± 6
61A1SP1390, or murBUDP-N-acetylmuramate dehydrogenase63 (5′)46 ± 15
30E4SP1492Putative cell wall surface anchor family protein16 (5′)49 ± 15
37C3 (2)SP1527, or aliBPutative oligopeptide ABC transporter75160 ± 23
20H6SP1531, or murEUDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate ligase45 (5′)55 ± 9
3F2SP1537General stress protein 1323841 ± 12
6H6SP1538Protein/peptidyl-prolyl cis-trans isomerase, Cof family78339 ± 7
32F5SP1570, or clpXATP-binding subunit of Clp protease14454 ± 18
27B9SP1586RNA helicase65347 ± 5
61D9SP1664, or ylmFConserved protein of unknown function33938 ± 4
61H10SP1682Putative sugar ABC transporter, permease86958 ± 13
6A3 (2)SP1687, or nanBNeuraminidase B74868 ± 15
69D7SP1731Conserved hypothetical protein5542 ± 9
29D5SP1745Isochorismate protein1567 ± 33
1D4SP1739Putative KH domain protein87851 ± 15
1C7 (2)SP1772Cell wall surface anchor family protein300 (5′)157 ± 48
35C7SP1903Conserved hypothetical protein21956 ± 15
9F3SP1939Putative MATE efflux family protein DinF124552 ± 16
12H7SP2131Putative transcriptional regulator of the BglG family657203 ± 43
27C9SP2190, or cbpACholine binding protein A26747 ± 4
22A4SP2192Sensor histidine kinase24644 ± 10
75H4SP2194, or clpCATP-binding subunit of Clp protease179349 ± 13
  • a Numbers in parentheses next to the mutant names refer to the number of transposon insertions isolated in that gene; the name of only one of the mutants is given.

  • b Gene names and assigned functions are from the TIGR4 genome annotation at the Comprehensive Microbial Resource site of the JCVI (http://cmr.jcvi.org/tigr-scripts/CMR/CmrHomePage.cgi).

  • c The transposon insertion position represents the number of nucleotides within the corresponding ORF or 5′ of the corresponding ORF (for insertions in promoters).

  • d Percentages of biofilm formation for each mutant relative to that of the wild type represent the means (±standard deviations) from four to five independent experiments.