Table 1.

Summary of experimental inoculation of newborn gnotobiotic piglets with spores of E. bieneusi isolates of human (H) or macaque (M) origin or after propagation in piglets (P)

No. of animalsInoculum (5 × 103 to 5 × 105)Immuno-suppressedOnset of spore/excretion (score)aEuthanatization point (days postchallenge)
4320/97M+6–8 (3+)9, 10, 20, 31
2320/97P+5–6 (3+)28
2114/97M+8 (3+)20
1FP/95Hb +6 (3+)12
4FP/95P+5 (3+)8, 14, 19
3JC/96H+6 (2+)16
4A57/97M+4–6 (2+)12, 17
3A57/97M9–12 (1+)14, 17
2Control+NA14, 50
4ControlNA21
  • a Maximum recorded shedding score over the observation period. 1+, <10 spores identified in the entire smear; 2+, spores readily found in most HP fields; 3+, >2 per HP field. NA, not applicable.

  • b “Isolate” FP/95H was actually a mixture of duodenal aspirates from two persons with AIDS designated BF/95H and WP/95H; these were mixed together because only small quantities of the material, stored over 2 years, were available.