Table 2.

A. marginale-specific IgG1 and IgG2 titers in the sera of calves immunized with outer membranes

Animal and serum sampleTiter (reciprocal of the dilution)a for:
IgG1IgG2
Immunized calves
 96BO5
  Preimmunization<10<10
  2 wk post-2nd inoculation5,0004,000
  1 mo postchallenge1,0001,000
  2 mo postchallenge5,0001,000
 96BO6
  Preimmunization<10<10
  2 wk post-2nd inoculation108,000
  1 mo postchallenge101,000
  2 mo postchallenge101,000
 96BO9
  Preimmunization<10<10
  2 wk post-2nd inoculation1016,000
  1 mo postchallenge<101,000
  2 mo postchallenge<101,000
Adjuvant controls
 96B19
  Preimmunization<10<10
  2 mo postchallenge100<10
 96B20
  Preimmunization<10<10
  2 mo postchallenge100<10
 96B21
  Preimmunization<10<10
  2 mo postchallenge<10<10
  • a Sera were diluted 1:10 to 1:32,000 and tested for reactivity against A. marginale Florida homogenate on Western blots. The titer is defined as the reciprocal of the highest dilution giving a positive signal on the blots. <10, no reaction at a dilution of 1:10 or higher.