Table 3.

Mean numbers of MLN cells and proportions of CD4+ and CD8+ T cells obtained from IL-10-deficient mice, wild-type mice, and heterozygous mice kept in either SPF or germfree conditionsa

Mouse groupNo. of MLN cells (106 [no. of mice])% CD4+ T cells (no. of mice)% CD8+ T cells (median)b (no. of mice)CD44 level (median)b, c (no. of mice)
SPF wild type22.7 ± 2.2 (29)36.6 ± 2.2 (15)15.4 ± 0.8 (15.3) (15)88.6 ± 5.99 (82.5) (12)
SPF IL-10 deficient33.4 ± 4.0* (25)28.4 ± 1.8* (12)22.8 ± 1.9 (24.1)** (12)130.3 ± 10.14 (127.5)** (14)
Germfree heterozygote9.5 ± 2.1 (6)36.7 ± 2.1 (7)21.4 ± 1.7 (21.1) (7)101 ± 4.56 (100) (7)
Germfree IL-10 deficient8.9 ± 1.2 (12)35.1 ± 1.6 (10)18.4 ± 1.6 (17.9) (10)97.5 ± 2.23 (98) (11)
  • a Except where indicated values are means ± standard errors of the mean. *, significantly different mean value compared to those for SPF wild-type, germfree heterozygote, and germfree IL-10-deficient mice (P < 0.05); **, significantly different median value compared to that for SPF wild-type mice (P < 0.05).

  • b Median values were used for statistical comparisons because of nonparametric data.

  • c Fluorescence intensity units.