Table 3.

Percentages of annexin V-positive, PI-negative (apoptotic) cells in unfractionated peripheral blood mononuclear cells and following purification of T cellsa

Cell typeMean % of annexin V+, PI cellsb ± SD following culture in medium with the indicated contents for:
72 h96 h
ControlToxin AControlToxin A
Unfractionated PBMCc 8.6 ± 2.746.7 ± 3.56.2 ± 2.953.6 ± 2.6
Purified T cells16.2 ± 4.639.3 ± 3.015.4 ± 2.749.2 ± 6.1
  • a Cells were isolated from five healthy volunteers and were cultured in control medium or with C. difficile toxin A (1,000 ng/ml) for 72 or 96 h before FACS analysis of gated lymphocytes.

  • b For control medium percentages versus toxin A percentages, for both unfractionated peripheral blood mononuclear cells and purified T cells, P < 0.001 at both 72 and 96 h.

  • c PBMC, peripheral blood mononuclear cells.