Table 3.

C. albicans strains constructed with the ura-blaster

StrainaSp actbFold reduction in enzyme activity compared to wild typecGene copy no.Doubling time in 2× YPD medium (h)dDoubling time in SD-ura medium (h)d
URA3Target gene
RSM312.87 ± 0.871.3211.41 ± 0.101.46 ± 0.01
RSM48.98 ± 0.441.9111.39 ± 0.04
RSM11 (SD-ura)8.75 ± 1.961.910
CAI4-018.28 ± 1.332.1111.27 ± 0.05
RSM118.11 ± 0.372.1101.38 ± 0.08
CWJ4307.85 ± 0.192.2111.23 ± 0.11
JKC197.53 ± 0.942.3100.90 ± 0.06
CAI4-107.49 ± 0.982.3111.34 ± 0.14
CACB8B-67.49 ± 0.732.3211.35 ± 0.151.42 ± 0.05
CACB8B-57.19 ± 0.132.4211.22 ± 0.05
CACB10B-86.46 ± 0.962.6111.25 ± 0.03
CAI4-1166.22 ± 0.372.7101.33 ± 0.11
CJC016.05 ± 0.972.8211.21 ± 0.10
RSM75.53 ± 1.013.1101.36 ± 0.111.70 ± 0.06
CAG14.55 ± 0.413.7111.23 ± 0.13
CJC0243.75 ± 0.204.5101.24 ± 0.08
CAG53.31 ± 0.385.1111.24 ± 0.10
CAI4-53.24 ± 0.605.2101.39 ± 0.072.11 ± 0.03
CACB10B-103.16 ± 0.415.4101.30 ± 0.12
CAG32.13 ± 0.108.0101.30 ± 0.08
CWJ4291.62 ± 0.7610111.15 ± 0.09
RSM171.23 ± 1.0618101.40 ± 0.061.87 ± 0.05
CAG40.56 ± 0.1130001.27 ± 0.05
  • a For genotype, see Table 1. Strains were grown in YPD medium unless otherwise noted.

  • b Reported as micromoles of OMP converted to UMP per minute per milligram of protein. Values are means ± standard deviations.

  • c For purposes of comparison, wild-type specific enzyme activity was 17.

  • d Reported as the averages of three independent cultures ± standard deviations.