Table 1.

Bacterial strains and plasmids

Strain or plasmidGenotype, serotype, or descriptionReference or source
E. coli strains
 XL1-Blue recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F′ proAB lacI q ZΔM15Tn10[Tetr])Stratagene
 S17-1 thi pro hsdR hsdM+recA::RP4-2Tc::Mu-Km::Tn7 52
Brucella strains
B. melitensis 16Ma Wild typeATCC 23456
B. ovis Reo 198a Rough, CO2independentBCCN R22 1
B. melitensis 16M Nalra Spontaneous nalidixic acid-resistant mutant 60
B. melitensis B115a Rough mutant, O side chain production in the cytoplasmBCCN R19 1, 16
B. melitensisH38Ra Rough mutant, no O side chain productionBCCN V3r 6, 14
B. melitensis B3B2Rough mini-Tn5 insertion mutantThis study
B. melitensis DR 1 to 4Rough double recombinantsThis study
Plasmids
 pUTmini-Tn5KmcatSuicide plasmid inBrucella spp. containing mini-Tn5Kmcat 20
 pBluescript KS(−)Phagemid cloning vector, Ampr Stratagene
 pBluescript SK(−) oriT Mobilizable phagemid cloning vector, Ampr,oriT 59
 pKSTn5RpBluescript KS derivative containing a 6.5-kb SalI chromosomal DNA fragment from the B3B2 insertion mutant; this fragment contains mini-Tn5 This study
 pSKoritTn5RpBluescript SK(−)oriT derivative containing a 6.5-kb SalI chromosomal DNA fragment from the B3B2 insertion mutant; this fragment contains mini-Tn5 This study
 pCAT19pUC19 containing a chloramphenicol acetyltransferase-encoding cassette 28
  • a This strain was received from J.-M. Verger (Laboratoire de Pathologie Infectieuse et d’Immunologie, Institut National de Recherche Agronomique, Nouzilly, France).