TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsReference or source
E. coli strains
    χ6212F λ φ80 Δ(lacZYA-argF) endA1 recA1 hsdR17 deoR thi-1 glnV44 gyrA96 relA1 ΔasdA426
    χ7213thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 ΔasdA4 recA129
S. enterica serovar Typhimurium strains
    χ3761UK-1 wild type7
    χ8879ΔasdA16 ΔphoP233 ΔsptP1033::xylE20
    χ9085ΔasdA16 ΔphoP233 ΔsptP1033::xylE hisG atrB13::MudJ20
    χ9242ΔphoP233 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBAD lacI TTThis study
Plasmids
    pYA3493T2SS signal sequence-based periplasmic secretion plasmid17
    pYA3658792 bp DNA encoding EAMZ250 in pYA365320
    pYA3700Cloning vector containing araC PBAD cassettepYA3698
    pYA3868Asd+; p15Aori; Pasd asd PsopE SD-(GTG)-sopE (N-terminal region)pYA3332
    pYA3870Asd+; p15Aori; Pasd asd PsopE SD-(ATG)-sopE (N-terminal region)pYA3332
    pYA4064Suicide vector harboring relA::araC PBAD lacI TTpRE112
    pYA4156740 bp DNA encoding the SO7 antigen in pYA3868pYA3868
    pYA4157740 bp DNA encoding the SO7 antigen in pYA3870pYA3870
    pYA4184740 bp DNA encoding the SO7 antigen in pYA3493pYA3493