TABLE 1.

Characterization of baboon θ-defensin sequences

BTDDeduced peptide sequence [precursor cDNA(s)]aChargeNative peptide massS-CAM peptide massd
Theor.bExptlcTheor.Exptl
1RCVCTRGFCRCVCRRGVC(a+b)+52,054.582,055.692,402.942,404.21
2RCVCRRGVCRCVCRRGVC(b+b)+62,061.612,062.722,409.972,411.73
3RCVCTRGFCRCVCTRGFC(a+a)+42,047.542,048.382,395.902,396.98
4RCVCTRGFCRCICLLGIC(a+c)+31,996.571,996.572,344.932,344.45
5RCVCRRGVCRCICLLGIC(b+c)+42,003.61NDNDND
6RCICLLGICRCICLLGIC(c+c)+21,945.61NDNDND
7RCVCTRGFCRCFCRRGVC(a+d)+52,102.622,103.702,450.982,452.29
8RCVCRRGVCRCFCRRGVC(b+d)+62,109.66NDNDND
9RCICLLGICRCFCRRGVC(c+d)+42,051.66NDNDND
10RCFCRRGVCRCFCRRGVC(d+d)+62,157.70NDNDND
  • a The letters in parentheses designate the two precursor cDNA(s) from which the mature peptide sequence was derived (Fig. 1). For example, the two precursor cDNAs for BTD-1 were BTD-a and BTD-b, which is shown as (a+b).

  • b Theor., theoretical. The calculated mass (Daltons) of the native or S-carboxamidomethylated (S-CAM) cyclic peptide is shown. ND, not determined.

  • c Determined by MALDI-TOF MS. ND, not determined.

  • d Isolated peptides were reduced with DTT and S-carboxamidomethylated (CAM) with iodoacetamide.