TABLE 2.

Isolation of RASV strains from chicken tissues 3 days following the first inoculation

Vaccine strainNo. of organisms isolated from tissues 3 days after immunizationa
SpleenBursaCecum
None (unimmunized)000
χ8879(pYA3870) (T3SS-vector control)1.9 ± 1.60 (1)b5.6 ± 0.937.2 ± 0.56
χ8879(pYA4157) (T3SS-SO7)0.7 ± 1.21 (2)5.4 ± 1.066.0 ± 0.50
χ9242(pYA4184) (T2SS-SO7)0.9 ± 1.61 (2)5.2 ± 0.386.8 ± 0.43
  • a Log10 geometric mean colony count per gram of tissue ± standard error obtained for three chickens. Groups of 21 chicks were inoculated when they were 7 days old with S. enterica serovar Typhimurium χ8879 (ΔsptP1033::xylE ΔasdA16 ΔphoP233) harboring plasmid pYA3870 or pYA4157 or with S. enterica serovar Typhimurium χ9242 (ΔphoP233 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBAD lacI TT) harboring pYA4184.

  • b The numbers in parentheses are the numbers of chicks with less than 10 CFU/g of tissue.