TABLE 2.

Summary of binding characteristics of Fn and Fg to the recombinant tandem repeat domains D1 to D5

Tandem repeat domain (immobilized amt [pg])Binding to binding partner:Net charge (% KR-EDb)
FnaFg (Kd [nM])
D1 (40)YesaYes (61.9)8 (7.4)
D2 (75)YesaYes (27)12 (11.1)
D3 (219)YesaYes (6.78)14 (13.6)
D4 (93)NoNo11 (10)
D5 (91)NoNo5 (5.2)
  • a No model available to fit the data. Fn showed the strongest binding to peptide D3. Fn at a concentration of 454 nM gave a signal of approximately 22 response units (RU) during binding to D1 (immobilized amount, 40 pg) and one of approximately 37 RU during binding to D2 (immobilized amount, 75 pg). However, Fn at a concentration of 477 nM gave a signal of approximately 280 RU during binding to D3 (immobilized amount, 219 pg).

  • b KR, lysine and arginine; ED, glutamic acid and aspartic acid.