Table 1.

Immune responses of mice 11 weeks after immunization by the i.v. route with different numbers of viable BCG

TreatmentMouseNo. of cytokine-producing cellsa/106 spleen cellsSerum antibody titerb
IFN-γIL-4IgG1IgG2a
Saline onlyC142200
C226400
C320200
C448200
BCG CFU
 ∼2 × 102 (low dose)L12924
L2980
L31016100100
L41880200
L59814200100
 ∼2 × 106(high dose)H12462228 × 1043.2 × 105
H22122784 × 1041.6 × 105
H33323028 × 1043.2 × 105
H43263004 × 1043.2 × 105
H53983708 × 1043.2 × 105
  • a As determined by antigen-dependent ELISPOT assay.

  • b Defined as the last dilution to give a positive result when tested by ELISA. All sera from mice before immunization had titers of <100 (—).