Table 1.

Primers used to amplify various regions of theeae genes from O127:H6 and O114:H2

pMal-Int constructPrimer
ForwardaReverseb
Int80α CTGAATTCGCCAGCATTACTGAGATTAAG CTTCTAGATTAGACATCGCTAACACGGGCAC
Int40-(1)α CTGAATTCGCCAGCATTACTGAGATTAAG CTTCTAGATTACGTCGTAAAGGTCACTTCCTG
Int40-(2)α CTGAATTCACGACCTTAGCTAAGTTAAGTAATCC CTTCTAGATTAGACATCGCTAACACGGGCAC
Int40-(3)α CTGAATTCTACACTGTTAAAGTGATGAAGGGG CTTCTAGATTATGTTACTTTGGCATAGCCATTCG
Int80β CTGAATTCGCCAGCATTACTGAGATTAAG CTTCTAGATTAACTCACTTTTGCACTAACAATG
Int130β CTGAATTCGCCAGCATTACTGAGATTAAG CTTCTAGATTAATTGCCCCCTGTTGTTGCCTGTAC
Int150β CTGAATTCAATGCAAAATACACATGGAAATCCAGTAAAAC CTTCTAGATTATTTTACACAARYKGCAWAAGC
Int100β CTGAATTCGGTACAGAAGTTAAGGCTACTACC CTTCTAGATTAACTACCCGGTGCATTTAATGTGT
Int120β CTGAATTCACAATTACTGTAGTATCTGGTGATC CTTCTAGATTATTTTACACAARYKGCAWAAGC
Int70β CTGAATTCTCAAAAGAACTATTGGCCATTATC CTTCTAGATTATTTTACACAARYKGCAWAAGC
Int40-(1)β CTGAATTCGCCAGCATTACTGAGATTAAG CTTCTAGATTACTTAGAAAAGGTCACTTTCTGATG
Int40-(2)β CTGAATTCAAGGATTTTGGGACCCTGAATAAG CTTCTAGATTAACTCACTTTTGCACTAACAATG
Int40-(3)β CTGAATTCTATACTGTCAGAGTGATGAAGG CTTCTAGATTATACAGTAGCATAACCATTCTCATCG
  • a Underlined sequences represent theEcoRI site.

  • b Underlined sequences represent theXbaI site.