Table 2.

Effects of α-mannosidases and proteinase K on adhesin activity of Fr.IIS

Enzymea% Adhesin activity of Fr.IIS (SD)b
α-Mannosidase (U)c
 0 (buffer only)90.7 (4.5)
 0.4471.0 (8.3)
 0.5543.2 (10.3)
 0.6623.1 (6.5)
 0.896.3 (9.8)
 1.131.8 (5.7)
α-1,2-Mannosidase (μU)d
  0 (buffer only)94.5 (2.3)
  0.583.2 (7.8)
  172.2 (11.5)
  546.9 (13.4)
 1041.3 (8.9)
 2030.2 (17.8)
Proteinase K (μg)
  0 (buffer only)87.4 (2.9)
 10083.3 (2.1)
  • a The indicated amount of each enzyme was added to 1 ml containing 200 μg of Fr.IIS. Samples were incubated with α-mannosidase or α-1,2-mannosidase for 18 h at 37°C or with proteinase K for 60 min at 37°C.

  • b Assessed by the ability of the fractions to block binding of C. albicans to the splenic marginal zone as described previously (17, 18).

  • c Isolated from C. ensiformis; releases α-1-2-, α-1-3-, and α-1-6-linked mannoses.

  • d Isolated from A. saitoi; releases α-1-2-linked mannoses at the nonreducing end.