Table 1.

Strains and plasmids used

Strain or plasmidReference or sourceDescription
Strains
 RN6390 18 Laboratory strain that maintains its hemolytic pattern when propagated on sheep erythrocytes and has a genetic background similar to that of 8325-4
 RN4220 21 Mutant of 8325-4 that accepts foreign DNA
 ALC1435This studyRN6390 with pALC1420 (sar P1 promoter)
 ALC1436This studyRN6390 with pALC1421 (sar P3 promoter)
 ALC1437This studyRN6390 with pALC1422 (sar P2 promoter)
 ALC1440This studyRN6390 with pALC1434 (vector)
Plasmids
 pCRIIInvitrogen E. coliPCR cloning vector
 pSK236 15 Shuttle vector containing pUC19 cloned into the HindIII site of pC194
 pSPT181 17 Shuttle vector
 pALC70 16 pSPT181 containing the entiresar locus with sarA, sarC, andsarB transcriptional units
 pALC1434This studyDerivative of pSK236 containing a promoterlessgfp UV gene preceded by a polylinker region
 pLC4 23 Transcriptional fusion vector containing a promoterless xylE reporter gene
 pALC669This studypCRII containing a 240-bp sarP1 promoter fragment (nucleotides 620 to 859) (2)
 pALC669This studypCRII containing a 162-bp sar P3 promoter fragment (nucleotides 364 to 525) (2)
 pALC678This studypCRII containing a 382-bp sar P2 promoter fragment (nucleotides 1 to 197 plus 185 bp upstream) (2)
 pALC1420This studypSK236 containing gfp UV preceded by the 240-bpsar P1 promoter fragment
 pALC1421This studypSK236 containing gfp UV preceded by the 162-bp sar P3 promoter fragment
 pALC1422This studypSK236 containing gfp UV preceded by the 382-bp sar P2 promoter fragment