Table 1.

Characterization of S. pneumoniae genes identified by STM

ClassStrainGeneaSimilar tob:% Amino acid identity (homology)FunctionRole in pathogenesisSepticemiac
Known virulence factorsSPN-673 yha Hydrolysis of hyaluronic acidHost colonization?High
SPN-605 nanA Hydrolysis of N -acetylneuraminic acidHost colonization?High
SPN-1264 lytA AutolysisRelease of pneumolysin and cell wall productsHigh
SPN-227CBP-likeUnknownUnknownMedium
SPN-1810 iga Cleavage of human IgA1Counteracts mucosal defense mechanismsLow
Metabolic functionsSPN-2078 purK (Bsu)48 (65)Purine biosynthesisIn vivo survival inSalmonella and Pseudomonas Low
SPN-786 purL (Bsu)24 (40)Purine biosynthesisIn vivo survival in Salmonella andPseudomonas Medium
SPN-1404 purE (Bsu)64 (78)Purine biosynthesisIn vivo survival in Salmonella andPseudomonas Medium
SPN-1645 purC (Bsu)54 (72)Purine biosynthesisIn vivo survival in Salmonella andPseudomonas Medium
SPN-181 adh(Bsu)34 (53)Anaerobic metabolismUnknownLow
SPN-902 glnA (Bsu)70 (82)Glutamine biosynthesisIn vivo survival in Salmonella Medium
SPN-1589 phoH (Bsu)62 (78)Phosphate assimilationUnknownHigh
ProteasesSPN-1055 clpC (Lmo)43 (65)ATP-dependent proteaseRequired for survival ofListeria in macrophagesMedium
ABC transportersSPN-1364 glnH (Bsu)33 (53)Glutamine transportIn vivo survival ofSalmonella Medium
SPN-1452 glnQ(Bsu)56 (76)Glutamine transportIn vivo survival ofSalmonella Medium
SPN-924 potD(Eco)41 (58)Spermidine/putrescine transportUnknownMedium
SPN-2041 potA(Eco)64 (75)Spermidine/putrescine transportUnknownLow
SPN-1802 msmK (Smsu)75 (84)Sugar transport?UnknownHigh
SPN-948 mesD(Lme)23 (44)UnknownUnknownHigh
DNA recombination/repairSPN-1411 recJ (Hin)34 (56)Methylation-directed mismatch repairSurvival to oxidative burst of macrophages?Low
SPN-1634 mutX Degradation of 8-oxo-dGTPSurvival to oxidative burst of macrophages?Low
  • a Gene already identified inS. pneumoniae.

  • b Determined by comparison of sequences and coding regions with the EMBL GenBank DNA, and SWISS-PROT databases. BLAST, BLASTX, and FASTA network services were used. Eco, Bsu, Hin, Lmo, Lme, Smu denote Escherichia coli, Lactococcus lactis, Bacillus subtilis, Haemophilus influenzae, Listeria monocytogenes, Leuconostoc mesenteroides, and Streptococcus mutans, respectively.

  • c For definitions of high, medium, and low, see Materials and Methods.