Table 1.

Characterization of S. pneumoniae genes identified by STM

ClassStrainGeneaSimilar tob:% Amino acid identity (homology)FunctionRole in pathogenesisSepticemiac
Known virulence factorsSPN-673yhaHydrolysis of hyaluronic acidHost colonization?High
SPN-605nanAHydrolysis of N -acetylneuraminic acidHost colonization?High
SPN-1264lytAAutolysisRelease of pneumolysin and cell wall productsHigh
SPN-227CBP-likeUnknownUnknownMedium
SPN-1810igaCleavage of human IgA1Counteracts mucosal defense mechanismsLow
Metabolic functionsSPN-2078purK (Bsu)48 (65)Purine biosynthesisIn vivo survival inSalmonella and PseudomonasLow
SPN-786purL (Bsu)24 (40)Purine biosynthesisIn vivo survival in Salmonella andPseudomonasMedium
SPN-1404purE (Bsu)64 (78)Purine biosynthesisIn vivo survival in Salmonella andPseudomonasMedium
SPN-1645purC (Bsu)54 (72)Purine biosynthesisIn vivo survival in Salmonella andPseudomonasMedium
SPN-181adh(Bsu)34 (53)Anaerobic metabolismUnknownLow
SPN-902glnA (Bsu)70 (82)Glutamine biosynthesisIn vivo survival in SalmonellaMedium
SPN-1589phoH (Bsu)62 (78)Phosphate assimilationUnknownHigh
ProteasesSPN-1055clpC (Lmo)43 (65)ATP-dependent proteaseRequired for survival ofListeria in macrophagesMedium
ABC transportersSPN-1364glnH (Bsu)33 (53)Glutamine transportIn vivo survival ofSalmonellaMedium
SPN-1452glnQ(Bsu)56 (76)Glutamine transportIn vivo survival ofSalmonellaMedium
SPN-924potD(Eco)41 (58)Spermidine/putrescine transportUnknownMedium
SPN-2041potA(Eco)64 (75)Spermidine/putrescine transportUnknownLow
SPN-1802msmK (Smsu)75 (84)Sugar transport?UnknownHigh
SPN-948mesD(Lme)23 (44)UnknownUnknownHigh
DNA recombination/repairSPN-1411recJ (Hin)34 (56)Methylation-directed mismatch repairSurvival to oxidative burst of macrophages?Low
SPN-1634mutXDegradation of 8-oxo-dGTPSurvival to oxidative burst of macrophages?Low
  • a Gene already identified inS. pneumoniae.

  • b Determined by comparison of sequences and coding regions with the EMBL GenBank DNA, and SWISS-PROT databases. BLAST, BLASTX, and FASTA network services were used. Eco, Bsu, Hin, Lmo, Lme, Smu denote Escherichia coli, Lactococcus lactis, Bacillus subtilis, Haemophilus influenzae, Listeria monocytogenes, Leuconostoc mesenteroides, and Streptococcus mutans, respectively.

  • c For definitions of high, medium, and low, see Materials and Methods.