Table 1.

Sequences of G3PDH- and cytokine-specific amplimers and expected sizes after amplification

Specific cytokineStrain specificityaSequence of oligonucleotideExpected size (bp) after amplification
IFN-γ+TGC ATC TTG GCT TTG CAG CTC TTC CTC ATG GC365
TGG ACC TGT GGG TTG TTG ACC TCA AAC TTG GC
TNF-α+TTC TGT CTA CTG AAC TTC GGG GTG ATC GGT CC354
GTA TGA GAT AGC AAA TCG GCT GAC GGT GTG GG
IL-4+CCA GCT AGT TGT CAT CCT GCT CTT CTT TCT CG357
CAG TGA TGT GGA CTT GGA CTC ATT CAT GGT GC
IL-10+ATG CAG GAC TTT AAG GGT TAC TTG GGT T455
ATT TCG GAG AGA GGT ACA AAC GAG GTT T
IL-12 (p40)+CCA CTC ACA TCT GCT GCT CCA CAA G266
ACT TCT CAT AGT CCC TTT GGT CCA G
MIP+ATG AAG GTC TCC ACC ACT GCC279
TCA GGC AAT CAG TTC CAG TTC CAG GTC AGT GAT GTA TTC
G3PDH+ACC ACA GTC CAT GCC ATC AC450
TCC ACC ACC CTG TTG CTG TA
  • a +, positive-sense strand; −, negative-sense strand.