Table 1.

Bacterial strains and plamsids used in this study

Strain or plasmidGenotype or descriptionSource or reference
Strains
E. coli
  DH5α recA BRL
  S17.1λpir RP4-2 Tc::Mu-Km::Tn7 21
S. dublin
  2229Wild-type, virulent field isolate of S. dublin IAHa
  B1 sipB::pDM4, Cmlr 29
  B2ΔsipB This study
  SB2ΔsopB 4
  B2SD1ΔsipB sopD::pDM4, Cmlr This study
  SD1 sopD::pDM4, Cmlr This study
  SB2SD1ΔsopB sopD::pDM4, Cmlr This study
  SpS spaS::pDM4, Cmlr This study
S. typhimurium12/75Virulent field isolateIAH
S. pullorum 449/87Virulent field isolateIAH
S. choleraisuis A57Virulent field isolateIAH
S. enteritidis 149Virulent field isolateIAH
S. gallinarum287/91Virulent field isolateIAH
Shigella sonnei PB1Virulent field isolateIAH
Plasmids
 pDM4oriR6K, Cmlr 15
 pBScya Ampr, pBluescript-basedcya gene fusion vectorK. Schesser
 pDelsipB1pDM4 vector containing the 5′ and 3′ flanking regions of sipB This study
 pSS1pDM4 vector containing XbaI-EcoRV PCR-derived insert ofspaS This study
 pMJ600 sopD-cyafusion plasmid, Ampr This study
 pMW45 sopD positive, isolated from a pBR322-basedSauIIIA library of S. dublin This study
  • a IAH, Institute for Animal Health.