Table 2.

Accumulation of cAMP, after 2 and 4 h, as an indication of sopD translocation by different S. dublin strainsa

StraincAMP produced (pmol) per well at:
2 h4 h
S. dublin22290.2 ± 0.080.50 ± 0.14
S. dublin2229(pMJ600)3.16 ± 1.163.5 ± 1.3
S. dublin B10.26 ± 0.230.45 ± 0.18
S. dublin B1(pMJ600)0.53 ± 0.090.5 ± 0.14
No infection0.55 ± 0.32<0.1
  • a HeLa cells were infected withS. dublin 2229, 2229(pMJ600), B1, and B1(pMJ600). A control of uninfected cells was used. The cAMP values (in picomoles ± standard deviations) are averages from three samples wells.