TABLE 5.

Cross-reactivity of two immunodominant Th1 T-cell epitopes of Ag85A, Ag85B, and Ag85C in DNA-vaccinated C57BL/6 micea

PeptideMean IL-2 (cpm) ± SDMean IFN-γ (pg/ml)
Ag85A DNAAg85B DNAAg85C DNAAg85A DNAAg85B DNAAg85C DNA
None (medium control)3,255 ± 1263,462 ± 6354,068 ± 4271243837
Ag85A 241-2608,632 ± 5264,988 ± 8954,947 ± 1751,45712,00452
Ag85B 240-26010,430 ± 11915,412 ± 1,2345,582 ± 761,23018,06919
Ag85C 241-2603,482 ± 7093,355 ± 8326,276 ± 2751458995
Ag85A 261-28013,534 ± 1,1466,194 ± 4816,451 ± 1927,4481,964365
Ag85B 262-27915,128 ± 1,39311,716 ± 5085,796 ± 7906,2082,414128
Ag85C 261-2805,672 ± 1884,523 ± 2415,175 ± 14360<1058
  • a IL-2 and IFN-γ levels in 24-h and 72-h spleen cell culture supernatants from C57BL/6 mice vaccinated with DNA encoding Ag85A, Ag85B, or Ag85C and restimulated in in vitro with medium or synthetic peptides covering overlapping regions of Ag85A, Ag85B, or Ag85C. Results are presented as mean values for pooled cells from four mice per group. Data from corresponding DNA and peptide pairs are indicated in bold.