TABLE 3.

Ag85-specific Th1-type cytokine secretion in BALB/c and C57BL/6 mice vaccinated with DNA encoding the three Ag85 components

MiceVaccinationMean IL-2 (cpm) ± SDaMean IFN-γ (pg/mL) ± SDb
Ag85A DNAAg85B DNAAg85C DNAAg85A DNAAg85B DNAAg85C DNA
BALB/cMedium2,595 ± 5123,752 ± 1,1182,939 ± 663<50<50<50
Ag85Ac22,090 ± 9,8985,784 ± 1,1184,005 ± 1,1461,092 ± 435293 ± 80142 ± 104
Ag85B4,742 ± 1,6578,519 ± 2,9723,202 ± 328266 ± 104472 ± 233142 ± 90
Ag85C7,301 ± 2,7804,306 ± 77010,307 ± 1,809523 ± 163482 ± 122396 ± 99
Ag8513,237 ± 4,0417,392 ± 1,3297,481 ± 1,560779 ± 78699 ± 284378 ± 93
C57BL/6Medium1,848 ± 2302,116 ± 4061,897 ± 232<50<50<50
Ag85A21,308 ± 1,56816,300 ± 4,7642,788 ± 5932,737 ± 2261,517 ± 462<50
Ag85B7,714 ± 2,05113,319 ± 4,7652,571 ± 2711,018 ± 4322,300 ± 711287 ± 191
Ag85C7,343 ± 3,4326,072 ± 1,7306,202 ± 23731,170 ± 661612 ± 1362,392 ± 713
Ag8516,487 ± 4,68712,860 ± 4,2974,252 ± 7552,300 ± 7001,926 ± 829934 ± 390
  • a Mean IL-2 levels measured in 24-h culture supernatant of spleen cells from mice vaccinated with DNA and unstimulated (medium) or restimulated in vitro with Ag85 components. Four mice were tested individually in each group. Data from corresponding DNA and antigen pairs are given in bold.

  • b Mean IFN-γ levels measured in 72-h culture supernatant of spleen cells from mice vaccinated with DNA and unstimulated (medium) or restimulated in vitro with Ag85 components. Four mice were tested individually in each group.

  • c Antigen used for in vitro restimulation (5 μg/ml).