TABLE 4.

Cross-reactivity of three immunodominant Th1 T-cell epitopes of Ag85A, Ag85B, and Ag85C in DNA-vaccinated BALB/c micea

PeptideMean IL-2 (cpm) ± SDMean IFN-γ (pg/ml)
Ag85A DNAAg85B DNAAg85C DNAAg85A DNAAg85B DNAAg85C DNA
None (medium control)5,422 ± 2293,868 ± 5604,699 ± 118627550
Ag85A 61-807,319 ± 955,955 ± 216,069 ± 6865901,863311
Ag85B 64-817,645 ± 6055,359 ± 4795,364 ± 6896741,670584
Ag85C 61-805,442 ± 654,352 ± 2615,591 ± 176713767720
Ag85A 101-12024,988 ± 9274,174 ± 2018,182 ± 132,07788235
Ag85B 100-1175,126 ± 50610,428 ± 1,4754,636 ± 4228341,058524
Ag85C 101-1207,432 ± 8215,024 ± 41223,463 ± 8433571063,822
Ag85A 191-21021,012 ± 7655,789 ± 5537,884 ± 949976143556
Ag85B 190-2078,637 ± 6755,648 ± 2995,596 ± 6051,00342129
Ag85C 191-2109,266 ± 6504,716 ± 5576,227 ± 23485355517
  • a IL-2 and IFN-γ levels in 24-h and 72-h spleen cell culture supernatants from BALB/c mice vaccinated with DNA encoding Ag85A, Ag85B, or Ag85C and restimulated in vitro with medium or synthetic peptides covering overlapping regions of Ag85A, Ag85B, or Ag85C. Results are presented as mean values for pooled cells from four mice per group. Data from corresponding DNA and peptide pairs are indicated in bold.