TABLE 2.

Oligonucleotides used in this study

PrimerSequence (5′ to 3′)a
TIR1CGCGGATCCGCGCCGTCTGTTTGTGAA
TIR2CCGGAATTCGGTAAAGGAGTGGATCCCA
TIR3CCGGAATTCAGTGATATCAAGGCTCTAA
TIR4CCGGAATTCAGGTCTCTATAGACGTTTAAA
TIR5CCGGAATTCGTTGGAAATACAGACATGCA
JFTIR1AATACAGACATGCATTTCTGTTATTTTGCTTG CATCAAAA
JFTIR3TTTTGATGCAAGCAAAATAACAGAAATGCAT GTCTGTATT
TF1CCGGAATTCTTGCAGACAATGTGCAGGAT
TF5CGCGGATCCATGCAAGCAAAATAACGCAC
BlueNotGGCCGCTCTAGAACTAGTG
  • a Restriction sites used in cloning are underlined.