TABLE 3.

Real-time PCRa of hemin-binding protein gene family in wild-type and hbpA mutant in B. quintana

Target geneWild typeMutantΔCTqc (avg)ΔΔCTdFold difference in mutant relative to wild type (2−ΔΔCT)
RNA amtb (ng)Avg CT ± SEMRNA amtb (ng)Avg CT ± SEM
16s rRNA1.2510.67 ± 0.111.2511.79 ± 0.251.12 (1.40)0.001.00
0.62512.00 ± 0.080.62513.35 ± 0.061.35
0.31312.86 ± 0.130.31314.60 ± 0.241.74
hbpC 1.2525.57 ± 0.151.2525.21 ± 0.11−0.36 (−0.40)−1.803.48 (increased in mutant)
0.62526.66 ± 0.110.62526.28 ± 0.15−0.38
0.31327.64 ± 0.180.31327.19 ± 0.13−0.45
hbpA 1.2522.08 ± 0.051.2525.27 ± 0.063.19 (3.13)1.73−3.33 (decreased in mutant)
0.62523.29 ± 0.040.62526.39 ± 0.073.10
0.31324.29 ± 0.020.31327.40 ± 0.043.11
hbpB 1.2530.02 ± 0.101.2529.77 ± 0.16−0.25 (−0.44)−1.843.58 (increased in mutant)
0.62531.25 ± 0.130.62530.68 ± 0.19−0.57
0.31332.58 ± 0.100.31332.08 ± 0.14−0.50
hbpD 1.2521.13 ± 0.051.2518.85 ± 0.02−2.28 (−2.38)−3.7813.74 (increased in mutant)
0.62522.02 ± 0.100.62519.78 ± 0.04−2.24
0.31323.44 ± 0.100.31320.81 ± 0.14−2.63
hbpE 1.2526.14 ± 0.061.2525.04 ± 0.01−1.10 (−1.17)−2.575.94 (increased in mutant)
0.62527.27 ± 0.020.62525.97 ± 0.05−1.30
0.31328.30 ± 0.100.31327.20 ± 0.06−1.10
  • a Reactions were performed in triplicate.

  • b That is, the nanogram amount of total RNA per 10-μl reaction.

  • c That is, the average mutant CT - the average wild-type CT. The average of all three values for each gene is given in parentheses.

  • d That is, the average ΔCTq minus the average ΔCTq for 16S rRNA.