TABLE 1.

Induction of the Shiga toxin receptor Gb3 on endothelial cellsa

Preincubation time (h)SMasebAmt of Gb3 (fmol/cell)Amt of Gb3/amt in control cells (%)
00.326100
0.25+0.644198
1.0+0.650199
4.00.289100
4.0+0.533191
  • a HDMEC were incubated with SMase (0.067 U/ml) for the times indicated, followed by extraction of the total amount of neutral glycosphingolipids and quantitative analysis of Gb3 by HPLC as described in Materials and Methods. These data are representative of results from duplicate experiments.

  • b +, SMase present; −, SMase not present.