Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
    B. quintana
        OK 90-268Human isolateCDCa
        LS100OK 90-268 transformed with pESTThis study
        LS200LS100 cured of pESTThis study
        LS300 hbpA in LS200 disrupted by pHBPA′This study
        LS400LS300 trans-complemented for hbpA using pBBR1-HBPAThis study
    E. coli DH5αHost strain for cloningGibco-BRL
    pESTReplicon for B. quintana 20
    pBluescript SK (+/−)Cloning vectorStratagene
    pBK-CMVExcised vector from λ ZapStratagene
    pUB1 Bartonella suicide vector 3
    pBBR1-MCS Bartonella shuttle vector 3
    pHBPA′Suicide vector-pUB1 containing hbpA′This study
    pBBR1-HBPApBBR1-MCS containing hbpAThis study
    pHBP-CMVpBK-CMV containing hbpA 6
    pHBPBpBluescript SK containing hbpBThis study
    pHBPCpBluescript SK containing hbpCThis study
    pHBPDpBluescript SK containing hbpDThis study
    pHBPEpBluescript SK containing hbpEThis study
  • a CDC, Centers for Disease Control and Prevention, Atlanta, Ga.