TABLE 2.

Vβ specificity of recombinant toxins on human PBLsa

Vβ cDNA% of Vβ enrichment after stimulation with:
ConASPE-LSPE-M
1.10.1 8.44 6.75
2.13.944.464.2
3.119.576.539.83
4.10.120.981.41
5.11.822.783.69
5.312.0610.213.96
5.83.314.494.43
6.31.031.572.15
6.40.631.411.47
6.95.964.854.92
7.30.840.941.1
7.42.311.711.47
8.10.30.680.79
9.11.341.782.05
12.315.367.768.93
12.51.171.381.94
14.10.30.790.95
15.12.263.924.25
17.1−0.071.051.12
18.11.512.543.13
22a1.12.092.27
23.10.451.391.41
    Total71.67882.2
  • a Human PBLs were incubated with recombinant toxin (20 pg/ml) for 4 days. Relative enrichment of Vβ cDNAs was analyzed from RNA of toxin-stimulated and ConA-stimulated PBLs by anchored-primer PCR and reverse dot blot to a panel of 22 different Vβ cDNAs. The figures represent the percentage of each Vβ with respect to total Cβ. Significant responses are underlined.