TABLE 1.

Genotyping of S. pyogenes isolates from New Zealanda

S. pyogenes isolateM/emmSiteDisease or conditionPCR result with primer for:
23SspeAspeCspeGbspeHbspeIspeJssaspeLspeMsmezb
FP 1943M53TSST+++++
FP 2658M59TSST+++++
FP 4223M80TSST+++++
FP 5417M41TSST++++++
FP 5971M57TSST+++++++
1/5045M4TSST+++++++
82/20M4SKUlcer+++++
85/167M12TSST++++++
85/314M89WSWound+++++
85/437M81WSInfections eczema++++++
85/723M22EarOtitis++++++
86/435M4TSRF++++++
87/19M12TSST++++++++
89/26M1TSAGN+++++
90/424M4TSST+++++++
94/229M49HVSEndometritis++++++c
94/712M89WSCellulitis+++++
95/31(2)M89WSAbscess++++
96/1M4HVSEndometritis++++++
9779emm56TSST+++++
9893M82TSST+++++++
10019emm44TSST+++++++
10303emm59TSST+++++
10438ST3018TSST+++++
10463emm49TSST++++++
10649ST2267TSST++++
10763M88TSST++++++
10791MNTTSST++++++++
10989M87TSST++++
11070emm65TSST++++c
11152M85TSST++++c
11222M92TSST++++++++
11227emm14TSST++++
11276MNTTSST++++++
11574ST809TSST++++
11646ST4547TSST+++++
11686M91TSST+++++
11789MNTTSST++++++
ATCCM1WoundND+++++++
33117T28R28NDSTSS+++++
    % With positive PCR result100314210026161003215592
Strain M89
85/314M89WSWound+++++
95/361M89PSAbscess++++++
82/675M89WSWound++++++
95/31M89WSAbscess++++
94/712M89WSCellulitis++++++
10846M89TSST++++++
89/54M89TSST++++++
94/11M89PSAbscess+++++
95/127M89BCCellulitis+++++
84/781M89TSST+++++
96/364M89BCBurns++++
    % With positive PCR result1002718100901000730100
  • a Fifty-one S. pyogenes isolates (38 from New Zealand, 1 from France, 1 ATCC reference strain, and 11 S. pyogenes M89 isolates collected in New Zealand) were genotyped. The results are based on PCR analysis using purified genomic DNA and specific primers for each sag gene. The primers for speL and speM were designed using the DNA sequences of the orthologous speLSe and speMSe genes from S. equi. Site and disease abbreviations are as follows: TS, throat site; WS, wound site; SK, skin; PS, pus site; HVS, high vaginal site; BC, blood culture; ST, sore throat; RF, rheumatic fever; AGN, acute glumerulonephritis; STSS, streptococcal toxic shock; ND, not determined.

  • b Reported previously (34).

  • c Positive by Southern blotting.