TABLE 1.

Antibody responses detected by ELISA and flow cytometry

Mouse strain and immunogenNo. of serum samples testedAvg antibody concn (μg/ml ± SD) detected by ELISAaSurface binding to EF3296
SW111Immunizing fragmentNo. of serum samples testedTimes control (avg ± SD)b
BALB/c
    None1c0.00 ± 0.00NDd51.00
    DHFR50.00 ± 0.000.00 ± 0.005ND
    SW11110507 ± 565507 ± 565169.25 ± 1.71
    HR10180.003 ± 0.0010.22 ± 0.13161.51 ± 0.09
    HR102837 ± 1988 ± 90166.36 ± 2.44
    HR10480.09 ± 0.0550 ± 25160.94 ± 0.03
    HR1071048 ± 1774 ± 76166.64 ± 0.80
    HR108823 ± 13225 ± 139162.24 ± 0.28
CBA/N
    None1c0.00 ± 0.00ND51.00
    DHFR50.00 ± 0.000.00 ± 0.005ND
    SW111843 ± 1443 ± 1485.47 ± 1.79
    HR10180.01 ± 0.0033.73 ± 5.0081.06 ± 0.06
    HR10212ND2.79 ± 5.07ND
    HR1078172 ± 118187 ± 11986.61 ± 1.15
    HR108811.3 ± 3.9189 ± 3886.81 ± 2.29
  • a Immune serum samples from BALB/c or CBA/N mice were tested for their anti-PspA antibody concentrations either against full-length α-helical PspA/EF3296 (SW111) or against the fragment used for immunization. Antibody concentrations and standard deviations were determined as described in Materials and Methods.

  • b Binding to the EF3296 bacterial surface was determined for each serum sample as the fluorescent signal of the sample divided by the fluorescent signal of a preimmune serum control.

  • c Pooled serum samples from five individual mice were used.

  • d ND, not done.