TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
    Y. enterocolitica
        WA-314Y. enterocolitica O:8 biotype 1B with pYV virulence plasmid25
        WA-CPlasmidless derivative of strain WA-314; spontaneously resistant to nalidixic acid25
        WA-CSDerivative of WA-C; spontaneous Smr mutantThis study
        8081Clinical isolate, O:8 biotype 1B40
        WA-CS yts1E::KanrDerivative of WA-CS; Nalr, Kanr Smr (insertional inactivation of yts1E by a kanamycin cassette)This study
        Ye1209-79Clinical isolate, O:13 biotype 1B25
        Ye1223-75-1Clinical isolate, O:20 biotype 1B25
        Ye737Clinical isolate, O:21 biotype 1B25
        Y-108Clinical isolate, O:3 biotype 425
        Y-96Clinical isolate, O:9 biotype 225
        NF-OClinical isolate, O:5 biotype 1A42
        MRS-40Clinical isolate, O:9 biotype 225
    Y. pseudotuberculosis
        H141/84Serotype O:1aS. Aleksic
        H457/86Serotype O:2aS. Aleksic
        IP3295Serotype O:1E. Carniel
        346Serotype O:3S. Aleksic
    Y. pestis
        KIMBiotype MediaevalisR. R. Brubaker
        KUMABiotype AntiquaR. R. Brubaker
        TSBiotype OrientalisR. R. Brubaker
    E. coli
        C-4441Enteroaggregative, O:12847
        12860Enteroinvasive, O:12447
        DH5αendA1 hsdR17 (rK mK+) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 (φ80lacZΔM15)23
        S17-1λpirpir+ tra+48
        XL1-Blue MRΔ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 laccStratagene
        MOSBlueendA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proA+B+ lacIqZΔM15:Tn10(Tcr)]Amersham
Plasmids
    Supercos1Cloning vector; Apr NeorStratagene
    ScF yts1E::KanrBglII/BamHI self-religated Supercos1 fragment carrying the mutated yts1E geneThis study
    pKAS 32Cloning vector with dominant rpsL gene49
    pKAS 32 yts1E::KanrpKAS32 carrying a fragment from the putative type II yts1E gene inactivated by a kanamycin cassetteThis study
    pACYC184Cloning vector; Cmr TcrNew England Biolabs
    pUC4KpUC vector carrying the kanamycin cassette from Tn903; Kanr AprAmersham
    pMOSBlueCloning vector; AprAmersham
  • a Nalr, nalidixic acid resistant; Tcr, tetracycline resistant; Cmr, chloramphenicol resistant.