TABLE 2.

Induction of immune effector mRNA in cultures of medium-rested control and BCG-expanded T cells incubated with infected macrophagesa

mRNARatio of T-cell cocultures
Control/inhibitoryInhibitory/control
TNF-α1.4 (1.1-3.7)<1
TGF-β1.3 (0.9-4)<1
IL-61.2 (0.4-9.7)<1
EGF2.2 (1.0-50)<1
IFN-γ<12.1 (0.9-3.5)
Granzyme A<16.9 (1.4-335)
Granzyme 3<12.2 (1.0-165)
Granulysin<16.5 (1.2-137)
Perforin<13.8 (1.2-156)
  • a Levels of expression of specific mRNA species detected by RNase protection assays were quantified by densitometry and normalized for levels of GADPH mRNA. Severalfold increases in mRNA present in cocultures with control T cells were calculated by dividing the normalized mRNA values detected in these cultures by the normalized mRNA values detected in cocultures with mycobacteria-specific inhibitory T cells. Severalfold increases in mRNA present in cocultures with mycobacteria-specific inhibitory T cells were calculated by dividing the normalized mRNA values detected in these cultures by the normalized mRNA values detected in cocultures with control T cells. Medians and ranges from four independent experiments studied after 24 h of coculture are presented.