TABLE 1.

Bacterial strains and plasmids

Strain or plasmidCharacteristicsReference or source
E. coli strains
    DH5αendA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [Φ80dlacΔ(lacZ)M15]48
    SM10 λpirpirR6K52
    HB101F Δ(gpt-proA)62 leu supE44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 xyl-5 mtl-1 recA1348
    1017HB101 ent::Tn57
S. flexneri strains
    SA100S. flexneri wild-type serotype 2a41
    SM100SA100 StrrS. Seliger
    SA240SA100 iucD::Tn527
    SM166SM100 sitA::camThis study
    SA167SA240 (iuc::Tn5) sitA::camThis study
    SA190SA100 feoB::dhfrThis study
    SM191SM166 (sitA::cam) feoB::dhfrThis study
    SA192SA190 (feoB::dhfr) iucD::Tn5This study
    SM193SA191 (sitA::cam feoB::dhfr) iucD::Tn5This study
    SA514SA100 Camr17
    SA101SA100 derivative with deletion in virulence plasmid6
    SA211SA101 fur::Tn551
Plasmids
    pEG2pLR29 carrying the sitA promoter47
    pHM5Allelic-exchange vector46
    pLAFR1Cosmid vector10
    pLR29pGTXN3 with RP4 mobilization region from pGP704 (35)47
    pWKS30Low-copy-number cloning vector54
    pEG1pLAFR1 carrying SA100 sit operonThis study
    pSIT1pLAFR1 carrying S. dysenteriae sit operon42
    pKL971pLAFR1 carrying SA100 iuc operon32