TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
    PA14Virulent burn wound isolate of P. aeruginosa47
    2B3PA14 pscD::TnphoAMiyata et al., unpublished
    2B3RReverted PA14 pscD::TnphoADrenkard and Ausubel, unpublished
    2B3R-restoredReverted PA14 pscD::TnphoA+ with a wild-type copy of pscDThis study
    ΔpscDPA14 ΔpscD (1.17-kb in-frame deletion of pscD)This study
    ΔexoTPA14 ΔexoT (1.3-kb in-frame deletion of exoT)This study
    ΔexoUPA14 ΔexoU (2.0-kb deletion of exoU)This study
    ΔexoYPA14 ΔexoY (1.1-kb deletion of exoY)This study
    ΔexoT ΔexoUPA14 ΔexoT ΔexoU (exoT and exoU deletions)This study
    ΔexoT ΔexoYPA14 ΔexoT ΔexoY (exoT and exoY deletions)This study
    ΔexoU ΔexoYPA14 ΔexoU ΔexoY (exoU and exoY deletions)This study
    ΔexoT ΔexoU ΔexoYPA14 ΔexoT ΔexoU ΔexoY (exoT, exoU, and exoY deletions)This study
    PA103Virulent lung isolate of P. aeruginosa2
    Sm10λpirE. coli strain used for mating constructs into P. aeruginosa; thi thr leu tonA lacY supE recA::RP4-2-Tc::mu Kmr15
    OP50E. coli strain used as a food source in C. elegans assays36
Plasmids
    pUCP19Cloning vector that replicates stably in Pseudomonas; Ampr50
    pEX18ApPositive selection suicide vector; Ampr45
    pEX18pscD1pEX18 with a 2.76-kb SacI-HindIII fragment containing pscD from PA14This study
    pEX18pscDΔ1pEX18 with a 1.17-kb KpnI-HindIII fragment containing an in-frame deletion of pscDThis study
    pEX18exoTΔ4pEX18 with a 1.3-kb KpnI-HindIII fragment containing an in-frame deletion of exoTThis study
    pEX18exoUΔ5pEX18 with a 2.0-kb KpnI-HindIII fragment containing a deletion of exoUThis study
    pEX18exoYΔ4pEX18 with a 1.1-kb EcoRI-HindIII fragment containing a deletion of exoYThis study