Strains and recombinant plasmids

Strain or plasmidDescriptionSource and/or reference
    E. coli DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsdR17(rK mK+) deoR thi-1 supE44 λ gyrA96 relA1; does not express AI-2GIBCO/Life Technologies
    V. harveyi
        BB170luxN::Tn5; AI-2 sensor strainV. Sperandio (22)
        BB152Positive control, synthesizes AI-2V. Sperandio (22)
    B. burgdorferi
        297Nonclonal, infectious human isolate12
        BbAH308Clonal, infectious luxS mutantThis study
        BbAH309Clonal, infectious luxS mutantThis study
    pGEM-TTA cloning vector, high-copy-number; AmprPromega
    pHAT20Expression vector with His tag; AmprClontech
    pALH133pGEM-T, ermC from pJRS233; Ampr Ermr8
    pALH330pGEM-T, 5,014-bp fragment (analogous B31 chromosome 385038-390052); AmprThis study
    pALH340pALH330, except luxS::ermC (BglII [1 kb]); Ampr ErmrThis study
    pALH470pHAT20Ω(luxS, AgeI/KpnI [560 bp]); AmprThis study