TABLE 1.

ORF-specific primers used to amplify fragments for recombinant expression

ORFSense primer(s)aAntisense primer(s)aAmplicon details
Size (bp)Portion of ORF
Tp01555′-ggatccaaccattgacacctgcc5′-gaattctgcagctgaattatagaac4285′ end
5′-ggatccttaacacgccgtcttcttc5′-gaattccggaagggtacgcatac6303′ end
Tp0316 (TprF)5′-ggatcctatgcaggcgtactcactccg5′-gaattctcagcaagcaccccctgttcc1,062Internal
Tp0326 (Tp92)5′-caggcaaacgacaattgg5′-caaattatttaccgtgaacg2,448Full length
Tp04535′-ggatcccgtggaaggcatcagtag5′-gaattccgaacttccctttttggag758Full length
Tp04835′-ggatccacgcggcgctcaaaaccg5′-gaattcgttatgaaagcgatagccg5903′ end
Tp05575′-ggatccatccgcacgtttttatccgcac5′-gaattcgggggcagtgtagcgcagg636Full length
Tp0620 (TprI)5′-ggatcccgactcaccctcgaacca5′-gaattcggtgagcaggtgggtgtag633Internal
Tp07515′-ggatccgggacaccgccgcacac5′-gaattccttgcggtgtgtgtgcgc3605′ end
Tp08565′-gcggcgaagactcgctc5′-cttgcgtccgagcagg1,086Full length
Tp09525′-ggatcctttccaagaagtcttcgaagtg5′-gaattcagcgcccccgttaaaggg5025′ end
5′-ggatcctgttgggacactcgatggg5′-gaattcattcgaaatgttttgctcacag5423′ end
S. aureus LBPb5′-ggatcctgccaattattacagatg5′-aagcttttatttatctaagttatagaa1,304Full length
  • a Restriction sites incorporated into the primers are underlined.

  • b LBP, laminin-binding protein.