TABLE 2.

ORFs up-regulated during DMC cultivation

ORF designation (description)RepliconFamilyCate- goryaSignal eptideFold up- regulation
BBQ01 (conserved hypothetical protein [Borrelia burgdorferi])Ip5655 familyHX6.78
BB0125 (hypothetical protein)ChromosomeU5.99
BBJ26 (ABC transporter, ATP-binding protein [Methanococcus jannaschii])lp384 familyTP5.48
BBB06 (PTS system, cellobiose-specific IIB component [cetA] [Bacillus subtilis])cp26TP4.39
BBG17 (hypothetical protein)Ip28-2U3.64
BBJ27 (hypothetical protein)lp38U3.59
BB0489 (ribosomal protein L24 [rplX] [Escherichia coli])ChromosomeRP3.53
BB0569 (hypothetical protein)ChromosomeU3.39
BB0603 (membrane-associated protein p66 [B. burgdorferi])ChromosomeCE+3.35
BBG19 (hypothetical protein)Ip28-2117 familyU3.32
BB0809 (tRNA-guanine transglycosylase [tgt] [Zymomonas mobilis])ChromosomeARS3.06
BB0844 (hypothetical protein)Chromosome12 familyU+3.02
BBQ63 (hypothetical protein, family 117 paralog, pseudogene)Ip56117 familyU2.95
BB0495 (ribosomal protein S5 [rpsE] [Bacillus stearothermophilus])ChromosomeRP2.92
BBA64 (antigen, P35 [B. burgdorteri])Ip5454 familyCE+2.73
BB0289 (flagellar assembly protein [fliH] [Borrelia burgdorferi])ChromosomeF2.64
BB0479 (ribosomal protein L4 [rplD] [B. burgdorferi])ChromosomeRP2.64
BB0298 (conserved hypothetical protein [B. burgdorferi])ChromosomeHX+2.63
BB0290 (flagellar motor switch protein [fliG-2] [B. burgdorferi])Chromosome38 familyF2.61
BB0364 (conserved hypothetical protein [Bacillus subtilis])ChromosomeHX2.56
BB0798 (competence protein F, putative [Haernophitus influenzae])ChromosomeX+2.43
BB0291 (flagellar basal-body rod protein [fliF] [B. burgdorferi])ChromosomeF2.26
BB0450 (RNA polymerase sigma-54 factor [ntrA] [Azotobacter vinelandii])ChromosomeTR2.23
BB0652 (protein-export membrane protein [secD] [Escherichia coli])Chromosome136 familyPE2.18
BBQ06 (conserved hypothetical protein [B. burgdorferi])Ip5648 familyHX2.16
BBG13 (hypothetical protein)Ip28-2U2.14
BB0419 (response regulatory protein [rrp-1] [Synechocystis sp. strain PCC6803])Chromosome14 familyGM2.08
BB0684 (carotenold biosynthesis protein, putative [Sulfolobus solfataricus])ChromosomeX2.02
BB0283 (flagellar hook protein [flgE] [B. burgdorferi])Chromosome78 familyF2.00
BBJ51 (vtsE1 protein, authentic frameshift [vlsE1] [B. burgdorferi])Ip38170 familyCE+2.00
BB0806 (hypothetical protein)ChromosomeU+1.99
BB0791 (thymidine kinase [tdk] [B. subtilis])ChromosomeNM1.96
BB0376 (S-adenosylmethionine synthetase [metK] [B. subtilis])ChromosomeIM1.94
BB0624 (hypothetical protein)ChromosomeU+1.94
BB0683 (3-hydroxy-3-methylglutaryl-CoA synthase [Arabidopsis thaliana])ChromosomeFM1.90
BB0393 (ribosomal protein L11 [rplK] [Thermotoga maritima])ChromosomeRP1.88
BBJ23 (hypothetical protein)Ip38106 familyU+1.85
BB0780 (ribosomal protein L27 [rpmA] [H. influenzae])ChromosomeRP1.83
BB0697 (conserved hypothetical protein [H. influenzae])ChromosomeHX1.77
BB0240 (glycerol uptake facilitator [glpF] [B. subtilis])ChromosomeTP1.75
BBG31 (conserved hypothetical protein [B. burgdorferi])Ip28-250 familyHX1.75
BB0440 (ribosomal protein L34 [rpmH] [B. burgdorferi])ChromosomeRP1.72
BB0640 (spermidine/putrescine ABC transporter, permease protein [potC] [E. coli])Chromosome41 familyTP1.71
BB0257 (cell division protein, putative [E. coli])ChromosomeD1.64
BB0559 (PTS system, glucose-specific IIA component [crr] [B. burgdorferi])ChromosomeTP1.63
BB0764 (sensory transduction histidine kinase, putative [B. subtilis])ChromosomeGM1.61
BB0449 (conserved hypothetical protein [E. coli])ChromosomeHX1.61
BBB19 (outer surface protein C (ospC) [B. burgdorferi])cp26CE+1.61
BB0036 (DNA topoisomerase IV [parE] [B. burgdorferi])Chromosome30 familyR1.59
BB0232 (hbbU protein [B. burgdorferi])ChromosomePD1.58
BB0712 (RNA polymerase sigma-70 factor [rpoD] [B. burgdorferi])Chromosome89 familyTR1.58
BBP34 (conserved hypothetical protein [B. burgdorferi])cp32-180 familyHX1.58
BB0675 (hypothetical protein)ChromosomeU1.56
BB0660 (GTP-binding protein [era] [E. coli])ChromosomeCH1.54
BB0538 (conserved hypothetical protein [B. burgdorferi])Chromosome125 familyHX1.52
BB0680 (methyl-accepting chemotaxis protein [mcp-4] [E. coli])Chromosome13 familyCH+1.51
BBG24 (hypothetical protein)Ip28-2104 familyU1.50
BBH36.2 (conserved hypothetical protein, pseudogene [B. burgdorferi])Ip28-3102 familyHX1.50
  • a ARS, amino acid biosynthesis; B, biosynthesis; CE, cell envelope; CH, chemotaxis proteins; D, division; F, flagellar biosynthesis; FM, fatty acid metabolism; GM, growth and metabolism; HE, hemolytic proteins; HS, heat shock proteins; HX, hypothetical conserved proteins; IM, intermediary metabolism; NM, nucleotide metabolism; PD, protein degradation; PE, protein export; PM, protein metabolism; R, replication; RP, ribosomal proteins; TF, translation factors; TP, transporter proteins; TR, transcriptional regulation proteins; U, hypothetical proteins; X, other.