TABLE 3.

Percentage of colonies obtained from outgrowth of frozen stocks of P1.1 and derivative cultures that have lost one or more of the plasmids shown during in vitro propagation as assessed by colony PCR

Temp (°C) and passagea% of colonies that lost:
lp25lp28-1lp28-4None
Passage 057124518
35°C
    Passage 33696614
    Passage 65711837
    Passage 2564151000
23°C
    Passage 333263733
    Passage 644115033
    Passage 25982072
  • a The number of colonies screened for passage 0 was 138 (the same inoculation culture was used for subsequent passages at 23 and 35°C), for passage 3 was 106 (35°C) and 46 (23°C), for passage 6 was 125 (35°C) and 46 (23°C), and for passage 25 was 138 (35°C) and 46 (23°C).