TABLE 1.

Ex vivo IL-4 production by PEC from BALB/c wt micea

Day postinfectionFraction% T cellsMean IL-4 concn (pg/ml) ± SD with:
Medium aloneMedium + ionomycin
NAb (PBS     control)Whole PEC43 ± 535 ± 47
7Whole PEC585 ± 23733 ± 58
Thy 1.2+17220 ± 1131,500 ± 0
Thy 1.20.320 ± 891 ± 33
14Whole PEC330 ± 13140 ± 26
Thy 1.2+1074 ± 1450 ± 71
Thy 1.20.313 ± 1480 ± 0
  • a PEC were recovered by peritoneal lavage from PBS-injected or L3-infected wt mice (n = 5) at days 7 and 14 postinfection. Equal total cell numbers of whole PEC, Thy 1.2-depleted, or Thy 1.2-enriched populations were cultured in the presence or absence of ionomycin as described in Materials and Methods. The cellular composition before and after separation was determined by CD3 staining for T cells and by intermediate Gr-1 staining and forward scatter-side scatter properties for eosinophils. IL-4 was measured from culture supernatants by sandwich ELISA as described in the text and is expressed as an average of multiple wells ± the standard deviation. (Six wells were analyzed for whole PEC samples, 11 wells for were analyzed Thy 1.2-depleted samples, and 2 wells were analyzed for Thy 1.2+ samples.)

  • b NA, not applicable.