TABLE 2.

Virulence of mutants of serovar Typhimurium C5 and serovar Gallinarum 9 for chickens and of serovar Typhimurium C5 and serovar Dublin 2229 for mice

Mutant geneNo. of animals that succumbed to salmonellosis/no. inoculated (%) [χ2 value; P value] usinga:
Newly hatched chickens, serovar Typhimurium C53-wk-old chickens, serovar Gallinarum 9Mice
Serovar Typhimurium C5Serovar Dublin 2229
None (parent)18/20 (90)16/20 (75)10/10 (100)7/9 (78)
nuoG6/20 (30) [χ2 = 15; P < 0.01]0/20 (0) [χ2 = 26.2; P < 0.01]8/10 (80) [χ2 = 2.22; P = 0.15]5/10 (50) [χ2 = 1.53; P = 0.4]
cydA2/20 (10) [χ2 = 40; P < 0.01]6/20 (30) [χ2 = 10.0; P < 0.01]6/10 (60) [χ2 = 5.0; P = 0.03]7/10 (70) [χ2 = 0.17; P = 0.6]
cyoA14/20 (70) [χ2 = 2.5; P = 0.03]4/20 (20) [χ2 = 14.4; P < 0.01]7/10 (70) [χ2 = 3.53; P = 0.6]8/10 (80) [χ2 = 0.01; P = 0.85]
atpB0/20 (< 10) [χ2 = 32; P < 0.01]1/15 (7) [χ2 = 18.5, P < 0.01]0/10 (0) [χ2 = 20; P < 0.01]0/10 (0) [χ2 = 12.45; P < 0.01]
atpH0/10 (< 10) [χ2 = 32; P < 0.01]Not done [not done]Not done [not done]Not done [not done]
  • a Chickens were inoculated orally within 24 h of hatching with 0.1 ml of bacterial culture containing approximately 3 × 108 CFU (serovar Typhimurium) or with 0.3 ml of culture containing approximately 109 CFU (serovar Gallinarum). Morbidity and mortality were recorded over 3 weeks, and χ2 comparisons were carried out. Six-week-old BALB/C mice were inoculated orally with 5 × 107 CFU in 50 μl of culture; morbidity and mortality were recorded over 2 weeks.