TABLE 3.

ORFs down-regulated during DMC cultivation

ORF designation (description)RepliconFamilyCate- goryaSignal peptideFold down- regulation
BBJ09 (outer surface protein D [ospD] [B. burgdorferi])lp38CE+−18.77
BBH16 (hypothetical protein)lp28-3U+−12.81
BB102 (conserved hypothetical protein [B. burgdorferi])lp28-484 familyHX−11.78
BBA62 (lipoprotein [B. burgdorferi])lp54CE+−11.73
BBI39 (hypothetical protein)lp28-454 familyU+−11.56
BBD18 (hypothetical protein [B. burgdorferi])lp17U−11.17
BBJ41 (antigen, P35, putative [B. burgdorferi])lp3854 familyCE+−10.80
BBI36 (antigen, P35, putative [B. burgdorferi])lp28-454 familyCE+−9.53
BBA16 (outer surface protein B [ospB] [B. burgdorferi])lp5453 familyCE+−8.66
BBI38 (hypothetical protein)lp28-454 familyU+−7.54
BBA69 (hypothetical protein)lp5454 familyU+−6.65
BBA68 (hypothetical protein)lp5454 familyU+−6.57
BBH29 (conserved hypothetical protein [B. burgdorferi])lp28-349 familyHX−5.90
BBA15 (outer surface protein A [ospA] [B. burgdorferi])lp5453 familyCE+−5.85
BBA74 (outer membrane porin [oms28] [B. burgdorferi])lp54171 familyCE+−5.79
BBK45 (immunogenic protein P37, putative [B. burgdorferi])lp3675 familyCE+−5.68
BBA38 (hypothetical protein)lp54146 familyU−5.40
BBA61 (conserved hypothetical protein [Borrelia garinii])lp54HX−5.18
BBA59 (lipoprotein [B. burgdorferi])lp54CE+−5.16
BB0152 (glucosamine-6-phosphate isomerase [nagB] [Haemophilus influenzae])ChromosomeIM−4.68
BBA40 (hypothetical protein)lp54148 familyU−4.54
BBA39 (hypothetical protein)lp54147 familyU−4.16
BBQ15 (conserved hypothetical protein [B. burgdorferi])lp56107 familyHX−4.13
BBR28 (lipoprotein [lp] [B. burgdorferi])cp32-4113 familyHX+−3.90
BBF06 (conserved hypothetical protein [B. burgdorferi])lp28-157 familyHX−3.75
BBJ19 (conserved hypothetical protein [B. burgdorferi])lp3862 familyHX−3.74
BBL29 (conserved hypothetical protein [B. burgdorferi])cp32-8161 familyHX−3.72
BBK13 (conserved hypothetical protein [Synechocystis sp. strain PCC6803])lp3640 familyHX+−3.70
BBP29 (conserved hypothetical protein [B. burgdorferi])cp32-1161 familyHX−3.68
BBK39 (hypothetical protein)lp3659 familyU−3.42
BBP22 (conserved hypothetical protein [B. burgdorferi])cp32-1142 familyHX−3.26
BBE19 (plasmid partition protein, putative [Bacillus subtilis])lp2532 familyHX−3.16
BBN27 (conserved hypothetical protein [B. burgdorferi])cp32-980 familyHX−3.12
BBA56 (hypothetical protein)lp54160 familyU−3.08
BBH15 (hypothetical protein)lp28-3U−2.87
BBR27 (conserved hypothetical protein [B. burgdorferi])cp32-480 familyHX−2.84
BBA52 (outer membrane protein [B. burgdorferi])lp54CE+−2.78
BBN22 (conserved hypothetical protein, authentic frameshift [B. burgdorferi])cp32-9142 familyHX−2.72
BBL28 (lipoprotein [B. burgdorferi])cp32-8113 familyCE+−2.62
BBR31 (conserved hypothetical protein [B. burgdorferi])cp32-457 familyHX−2.60
BBS31 (conserved hypothetical protein [B. burgdorferi])cp32-3161 familyHX−2.59
BBO27 (conserved hypothetical protein [B. burgdorferi])cp32-780 familyHX−2.54
BBF09 (hypothetical protein, paralogous family 71, authentic frameshift)lp28-171 familyU−2.40
BBK07 (hypothetical protein)lp3659 familyU+−2.40
BBP30 (conserved hypothetical protein [B. burgdorferi])cp32-157 familyHX−2.33
BBJ31 (hypothetical protein)lp3859 familyU−2.23
BBA55 (hypothetical protein)lp54159 familyU−2.18
BBA73 (antigen, P35, putative [B. burgdorferi])lp5454 familyCE+−2.14
BBJ08 (hypothetical protein)lp3812 familyU+−2.09
BB0248 (oligoendopeptidase F [pepF] [Lactococcus lactis])ChromosomePD−2.08
BBS25 (conserved hypothetical protein [B. burgdorferi])cp32-3112 familyHX−2.05
BBE23.2 (hypothetical protein, authentic point mutation [B. burgdorferi])lp2532 familyU−2.05
BBR34 (conserved hypothetical protein [B. burgdorferi])cp32-449 familyHX−2.05
BBN21 (hypothetical protein, paralogous family 141, authentic frameshift [B. burgdorferi])cp32-9141 familyU−2.04
BBJ36 (hypothetical protein)lp3892 familyU+−1.99
BBP28 (lipoprotein [B. burgdorferi])cp32-1113 familyCE+−1.94
BBL15 (hypothetical protein)cp32-8156 familyU−1.91
BBN29 (hypothetical protein, paralogous family 161, authentic point mutation [B. burgdorferi])cp32-9161 familyU−1.82
BBF26.1 (conserved hypothetical protein, pseudogene [B. burgdorferi])lp28-1101 familyHX−1.81
BB0757 (ATP-dependent Clp protease proteolytic component [clpP-2] [H. influenzae])Chromosome3 familyPD−1.78
BBN04 (hypothetical protein)cp32-9148 familyU−1.78
BBO25 (conserved hypothetical protein [B. burgdorferi])cp32-7112 familyHX−1.77
BBR04 (hypothetical protein)cp32-4148 familyU−1.77
BBP13 (hypothetical protein)cp32-1154 familyU−1.75
BBR25 (conserved hypothetical protein [B. burgdorferi])cp32-4112 familyHX−1.61
BBQ32 (conserved hypothetical protein [B. burgdorferi])lp56112 familyHX−1.55
BBS09 (conserved hypothetical protein [B. burgdorferi])cp32-3108 familyHX−1.55
  • a ARS, amino acid biosynthesis; B, biosynthesis; CE, cell envelope; CH, chemotaxis proteins; D, division; F, flagellar biosynthesis; FM, fatty acid metabolism; GM, growth and metabolism; HE, hemolytic proteins; HS, heat shock proteins; HX, hypothetical conserved proteins; IM, intermediary metabolism; NM, nucleotide metabolism; PD, protein degradation; PE, protein export; PM, protein metabolism; R, replication: RP, ribosomal proteins; TF, translation factors; TP, transporter proteins; TR, transcriptional regulation proteins; U, hypothetical proteins; X, other.