TABLE 2.

Toxin recovery from mouse feces after bacterial challenge

Bacterial challengeaNo. of toxin-positive miceb/total mice (positive values [ng/ml])
Single-infection groups
    109 C600K0/12
    109 C600G::Δtox0/12
    107 C600A::933W5/18 (106, 42, 39, 37, 13)
Coinfection groups
    109 C600K + 107 C600A::933W7/37 (2140, 1350, 100, 70, 16, 15, 13)c
    109 C600G::Δtox + 107 C600A::933W1/37 (17)d
  • a Mice were inoculated intragastrically with one or two strains. After 24 h, all of the feces from each mouse were collected and assayed for bacterial and toxin recovery.

  • b The limit of detection for fecal Stx2 was 5 ng/ml.

  • c Statistically different toxin recovery (P < 0.05 [Student t test of log toxin values]) compared to toxin from the doubly infected C600G::Δtox and C600A::933W group, determined by using the values for all of the mice in each group.

  • d Statistically different toxin recovery (P < 0.05, Student t test of log toxin values) compared to toxin from the C600A::933W group, determined by using the values for all of the mice in each group.